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Gopala Reddy, A.
- Protective Effect of Curcumin Against Imidacloprid-Induced Genotoxicity in Rats
Authors
1 Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science, Hyderabad-500030, Telangana, IN
Source
Toxicology International (Formerly Indian Journal of Toxicology), Vol 22, No 2 (2015), Pagination: 65-69Abstract
Aim: To study the effect of curcumin in imidacloprid (IMI)-induced genotoxicity by comet and micronucleus assays.
Materials and Methods: Experiments were conducted on adult rats that were randomly divided into six groups of six rats as follows: Group 1: Sham; Group 2: Curcumin (50 mg/kg po); Group 3: IMI (20 mg/kg po); Group 4: IMI (40 mg/kg po); Group 5: IMI (20 mg/kg po) + curcumin (50 mg/kg po); and Group 6: IMI (40 mg/kg po) + curcumin (50 mg/kg po). Micronucleus and comet assays were used to study the DNA damage in all the groups.
Results: There was a significant (P < 0.05) increase in the comet tail length (μm) and the frequency of micronuclei formation in the Group 4 treated with 40 mg/kg IMI when compared to other groups. Curcumin treatment in Group 6 showed a significant (P < 0.05) decrease in the comet tail length, and micronuclei as compared to Group 4 though the decrease was significantly (P < 0.05) low as compared to other groups.
Conclusions: This study revealed that IMI has a tendency to induce the genotoxicity on exposure to 40 mg/kg b.wt. Curcumin, due to its anti-oxidant and anti-mutagenic property, alleviated the genotoxicity induced by IMI.
Keywords
Comet Assay, Curcumin, DNA, Genotoxicity, Imidacloprid.References
- Tomizawa M, Casida JE. Neonicotinoid insecticide toxicology: Mechanisms of selective action. Annu Rev Pharmacol Toxicol. 2005; 45:247–68.
- Tomizawa M, Casida JE. Selective toxicity of neonicotinoids Devi, et al.: Curcumin against imidacloprid-induced genotoxicity attributable to specificity of insect and mammalian nicotinic receptors. Annu Rev Entomol 2003;48:339–64.
- Matsuda K, Buckingham SD, Kleier D, Rauh JJ, Grauso M, Sattelle DB. Neonicotinoids: Insecticides acting on insect nicotinic acetylcholine receptors. Trends Pharmacol Sci. 2001; 22:573– 80.
- Chauzat MP, Carpentier P, Martel AC, Bougeard S, Cougoule N, Porta P, et al. Influence of pesticide residues on honey bee (Hymenoptera: Apidae) colony health in France. Environ Entomol. 2009; 38:514–23.
- Abou-Donia MB, Goldstein LB, Bullman S, Tu T, Khan WA, Dechkovskaia AM, et al. Imidacloprid induces neurobehavioral deficits and increases expression of glial fibrillary acidic protein in the motor cortex and hippocampus in offspring rats following in utero exposure. J Toxicol Environ Health A .2008; 71:119–30.
- Karabay NU, Oguz MG. Cytogenetic and genotoxic effects of the insecticides, imidacloprid and methamidophos. Genet Mol Res. 2005; 4:653–62.
- Bal R, Turk G, Tuzcu M, Yilmaz O, Kuloglu T, Gundogdu R, et al. Assessment of imidacloprid toxicity on reproductive organ system of adult male rats. J Environ Sci Health B. 2012; 47:434–44.
- Kulkarni SK, Dhir A. An overview of curcumin in neurological disorders. Indian J Pharm Sci. 2010; 72:149–54.
- Kulkarni S, Dhir A, Akula KK. Potentials of curcumin as an antidepressant. Scientific World Journal. 2009; 9:1233– 41.
- Asare GA, Bugyei K, Sittie A, Yahaya ES, Gyan B, Adjei S, et al. Genotoxicity, cytotoxicity and toxicological evaluation of whole plant extracts of the medicinal plant Phyllanthus niruri (Phyllanthaceae). Genet Mol Res. 2012; 11:100–11.
- Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res. 1988; 175:184–91.
- Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H, et al. Single cell gel/comet assay: Guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen. 2000; 35:206–21.
- Feng S, Kong Z, Wan X, Peng P, Zeng EY. Assessing the genotoxicity of imidacloprid and RH-5849 in human peripheral blood lymphocytes in vitro with comet assay and cytogenetic tests. Ecotoxicol Environ Saf. 2005; 61:239–46.
- Carmello JC, Pavarina AC, Oliveira R, Johansson B. Genotoxic effect of photodynamic therapy mediated by curcumin on Candida albicans. FEMS Yeast Res. 2015; 15:fov018.
- Biswas J, Roy S, Mukherjee S, Sinha D, Roy M. Indian spice curcumin may be an effective strategy to combat the genotoxicity of arsenic in Swiss albino mice. Asian Pac J Cancer Prev. 2010; 11:239–47.
- Majer BJ, Laky B, Knasmuller S, Kassie F. Use of the micronucleus assay with exfoliated epithelial cells as a biomarker for monitoring individuals at elevated risk of genetic damage and in chemoprevention trials. Mutat Res. 2001; 489:147–72.
- Modulation of Oxidative Stress Mediators in Adjuvant Induced Arthritis by Herbal Adaptogen Boswellia serrata
Authors
1 JRF, ICAR-NDRI, Southern Regional Station, Adugodi, Bengaluru – 560030, Karnataka, IN
2 Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science, Rajendranagar, Hyderabad – 500030, Telangana, IN
3 Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science, Korutla, Kanchikacherla - 505326, Telangana, IN
Source
Toxicology International (Formerly Indian Journal of Toxicology), Vol 25, No 4 (2018), Pagination: 193-197Abstract
A study was carried out to evaluate Boswellia serrata on adjuvant induced oxidative stress in rats. Intradermal administration of Freund’s adjuvant to induce rheumatoid arthritis consequently produced oxidative damage as indicated by an increase in thiobarbituric acid reducing substances, myeloperoxidase, protein carbonyls and a reduction in superoxide dismutase, catalase, glutathione peroxidase and reduced glutathione in tissue homogenates. Oral administration of methanolic extracts ofBoswellia serrata at 500 mg/kg body weight re-established the antioxidant profile. Co-treatment with an anti-inflammatory agent, meloxicam showed an enhanced healing effect. Hence, a significant restorative effect was witnessed with the herbal adaptogen Boswellia serrata.Keywords
Adjuvant Arthritis, Antioxidants, Boswellia serrata, Oxidative Stress.References
- Stills HF, Bailey MQ. The use of Freund’s complete adjuvant. Lab Animal Science 1991; 20:25–31 2. Cho ML, Kim WU, Min SY, Min DJ, Min JK, Lee SH. Cyclosporine differentially regulates interleukin-10, interleukin-15, and tumour necrosis factor α production by rheumatoid synoviocytes.Arthritis Rheum.. 2002; 46:42–51 https://doi.org/10.1002/1529-0131(200201)46:1<42::AIDART10026>3.0.CO;2-A.
- Mishafiey A, Cuzzocreay SB, Mazzony E, Saadat F, Stoude M. Treatment of experimental arthritis with M2000, a novel designed non-steroidal anti-inflammatory drug. Scand. J. Immunol. 2005; 61:435–41. https://doi.org/10.1111/j.13653083.2005.01594.x.PMid:15882435.
- Tak PP, Zvaifler NJ, Green DR, Firestein GS. Rheumatoid arthritis and p53: How oxidative stress might alter the course of inflammatory diseases. Immunol Today. 2000; 21:78–2. https://doi.org/10.1016/S0167-5699(99)01552-2.
- Hitchon CA, El-Gabalawy HS. Oxidation in rheumatoid arthritis. Arthritis Research and Therapy 2004; 6:265– 78. https://doi.org/10.1186/ar1447. PMid:15535839. PMCid:PMC1064874
- Cope AP. Studies of T-cell activation in chronic inflammation. Arthritis Research. 2002; 4:197–211. https://doi.org/10.1186/ar557. PMCid:PMC3273158
- Gupta I, Parihar A, Malhotra P, Gupta S, Ludtke A, Safayhi H, Ammon HPT. Effects of gum resin of Boswellia serrata in patients with chronic colitis. Planta Med. 2001; 67:391–5. https://doi.org/10.1055/s-2001-15802. PMid:11488449
- Pandey RS, Singh BK, Tripathi YB. Extract of gum resins of Boswellia serrata inhibits lipopolysaccharide induced nitric oxide production in rat macrophages along with hypolipidemic property. Indian J. Exp. Biol.. 2005; 43:509–16.
- Ross RMD. Therapeutic advantages of highly standardized Boswellia extracts. True Botanica. 2008; 1–37
- Balasubramanian KA, Manohar M, Mathan VI. An unidentified inhibitor of lipid peroxidation in intestinal mucosa. Biochim Biophys Acta. 1988; 96:51–8. https://doi.org/10.1016/0005-2760(88)90094-X
- Krawisz JF, Sharon P, Stenson WF. Quantitative assay for acute intestinal inflammation based on myeloperoxidase activity: Assessment of inflammation in rat and hamster models. Gastroenterology 1984; 87:1344–50. https://doi.org/10.1016/0016-5085(84)90202-6.
- Levine JD, Goetzl EJ, Basbaum AI. Contribution of the nervous system to the pathophysiology of rheumatoid arthritis and other polyarthritis. Rheumatic Disease of Clinics of North America. 1987; 13:369–83.
- Madesh M, Balasubramanian KA. Microtiter plate assay for superoxide dismutase using MTT reduction by superoxide. Indian Journal of Biochemistry and Biophysics. 1998; 35:184–0.
- Asru KS. Colorimetric assay of catalase. Analytical Biochemistry. 1972; 47:389–94 https://doi.org/10.1016/0003-2697(72)90132-7.
- Moron MS, Depierre JW, Mannervik B. Levels of glutathione, glutathione reductase and glutathione S transferase in rat lung and liver. Biochem Biophys Acta. 1979; 582:67–8. https://doi.org/10.1016/0304-4165(79)90289-7.
- Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. Journal of Laboratory and Clinical Medicine. 1967; 70:158–69
- Storey K. Oxidative stress: animal adaptations in nature. Brazilian Journal of Medical and Biological Research 1996; 29: 1715–33
- Gambhir JK, Lali P, Jain AK. Correlation between blood antioxidant levels and lipid peroxidation in rheumatoid arthritis. Clinical Biochemistry. 1997; 30:351–5 https://doi.org/10.1016/S0009-9120(96)00007-0.
- Okabe T, Hamaguchi K, Inafuku T, Hara M. Aging and superoxide dismutase activity on cerebrospinal fluid. J. Neurol. Sci.. 1996; 141:100–4. https://doi.org/10.1016/0022510X(96)00160-8
- Hassan MQ, Hadi RA, Al-Rawi ZS, Padron VA, Stohs SJ. The glutathione defence system in the pathogenesis of rheumatoid arthritis. J Appl Toxicol. 2001; 21:69–73 https://doi.org/10.1002/jat.736.
- Alessio HM, Blasi ER. Physical activity as a natural antioxidant booster and its effect on healthy life style. Research Quarterly for Exercise and Sports. 1997; 68:292–302. https:// doi.org/10.1080/02701367.1997.10608010. PMid:9421841
- Darlington LG, Stone TW. Antioxidants and fatty acids in the amelioration of rheumatoid arthritis and related disorders. Br. J. Nutr. 2001; 85:251–69. https://doi.org/10.1079/BJN2000239.
- Visva KK, Premila A, Bina I. Alteration in antioxidant defence mechanisms in the small intestines of Methotrexate treated rat may contribute to its gastrointestinal toxicity. Cancer Therapy. 2007; 5:501–10.
- Siess H. Oxidative stress: From basic research to Clinical Application. Am J Med. 1991; 91:38–85. https://doi.org/10.1016/0002-9343(91)90281-2.
- Uhlig S, Wendal A. The physiological consequence of glutathione variations. Life Science. 1992; 51:1083–94. https:// doi.org/10.1016/0024-3205(92)90509-N.
- Kohen R, Nyska A. Oxidation of biological systems: Oxidative stress phenomena, antioxidants, redox reactions and methods for their quantification. Toxicol. Pathol. 2002; 30:620–50. https://doi.org/10.1080/01926230290166724. PMid:12512863
- Doha AM, Sahar YA. In vivo evaluation of antioxidant and anti-inflammatory activity of different extracts of date fruits in adjuvant arthritis. Pol. J. Food Nutr. Sci. 2004; 13:397–402.
- Ratheesh M, Shyni GL, Helen A. Methanolic extract of Ruta graveolens L. inhibits inflammation and oxidative stress in adjuvant induced model of arthritis in rats. Inflammopharmacology 2009; 17:100–5. https://doi.org/10.1007/s10787-009-8044-0. PMid:19205849
- Takeuchi K, Tanaka A, Ohno R, Yokota A. Role of COX inhibition in pathogenesis of NSAID-induced small intestinal damage. J Physiol Pharmacol. 2003; 54:165–82.
- Lisa KS, Irada K, Joanna MT, Revathy S, Rufus T, Richard CH, Paul GW, Anthony JK. Myeloperoxidase and oxidative stress in rheumatoid arthritis. Rheumatology. 2012; 51:1796–803. https://doi.org/10.1093/rheumatology/kes193. PMid:22814531
- Afsar V, Reddy M, Saritha KV. In-vitro anti-oxidant activity and anti-inflammatory activity of methanolic leaf extract of Boswellia serrata. International Journal of Biological and Pharmaceutical Research. 2012; 1:15–23.
- Chevion M, Berenshtein E, Stadtman ER. Human studies related to protein oxidation: Protein carbonyl content as a marker of damage. Free Radical Research. 2000; 33:99–108.
- Eduardo EMJ, Jose AOS, Andres C, Daniel CD, David J, Roberto G, Eduardo M, Cesar MA, Ronaldo HM, Enrique P. Piroxicam and meloxicam ameliorate hepatic oxidative stress and protein carbonylation in kupffer and sinusoidal endothelial cells promoted by ischemia-reperfusion injury. Transplant International. 2011; 24:489–500. https://doi.org/10.1111/j.1432-2277.2010.01214.x. PMid:21261753
- Study of Cadmium and Chlorpyrifos Induced Changes in Protein and Lipid Profile in Rats
Authors
1 Department of Veterinary Pathology, College of Veterinary Science, Rajendranagar, Hyderabad – 500030, Telangana, IN
2 Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science, Korutla, Jagtial District, Telangana – 505326, Telangana, IN
3 Department of Pharmacology and Toxicology, College of Veterinary Science, Rajendranagar, Hyderabad – 500030, Telengana, IN
Source
Toxicology International (Formerly Indian Journal of Toxicology), Vol 27, No 1&2 (2020), Pagination: 54-57Abstract
The present work was aimed to know the changes in protein and lipid profile induced cadmium and chlorpyrifos Wistar rats. Work was carried out for 28 days in rats. 1: Control. 2: CdCl2 @ 22.5 mg/kg b.wt/oral. 3: CPF @ 25 mg/kg b.wt/per oral. 4: CdCl2@22.5 mg + CPF @ 25 mg/kg b.wt/per oral. Results revealed a significant (P<0.05) decrease in the concentration of total protein and albumin in Groups 2, 3 and 4 and also a significant (P<0.05) increase in total cholesterol, triglycerides and phospholipids in Groups 2, 3 and 4 on 15th and 29th day of the experiment. The adverse changes in protein and lipid profile in combined Group were severe than individual Groups due to synergistic action of the combined pollutants.Keywords
Cadmium, Chlorpyrifos, Lipid Profile, Protein Profile, Wistar Rats.References
- Calderoni AM, Oliveros L, Jahn G, Anton R, Luco J and Gimenez. MS. Alterations in the lipid content of pituitary gland and serum prolactin and growth hormone in cadmium treated rats. Biometals. 2015; 18: 213–20. PMid: 15984566. https://doi.org/10.1007/s10534-005-0581-4
- Poulsen BM, Andersen HR, Grandjean P. Potential developmental neurotoxicity of pesticides used in Europe. J Environ Health. 2008; 7:50. PMid: 18945337 PMCid:
- PMC2577708. https://doi.org/10.1186/1476-069X-7-50 3. Limei Z, Xiaoyong L, Tongbin C, Xiulan,Y, Hua X, Bin W, Lixia W. Regional assessment of cadmium pollution in agricultural lands and the potential health risk related to intensive mining activities: A case study in Chenzhou City. J Environ Sci China. 2008; 20(6):696–703. https://doi. org/10.1016/S1001-0742(08)62115-4
- Yadala R, Madhuri D, Lakshman M, Gopala Reddy A, Kalakumar B. Haematological alterations induced by Cadmium (Cd) and Chlorpyrifos (CPF) in Male Wistar albino Rats. Int J Curr Microbiol App Sci. 2019; 8(08):480– 5. https://doi.org/10.20546/ijcmas.2019.808.055
- Henson MC, Chedrese PJ. Endocrine disruption by cadmium, a common environmental toxicant with paradoxical effects on reproduction. Exp Biol Med. 2004; 229(5):383–92. PMid: 15096650. https://doi. org/10.1177/153537020422900506
- Idris SB, Ambali SF, Ayo JO. Cytotoxicity of chlopyrifos and cypermethrin: The ameliorative effects of antioxidants. Afr J Biotechnol. 2012; 11(99):16461–7.
- Curcic M, Sasa J, Vesna J, Sanja V, Slavika V, Ksenija D, Zorica B, Biljana A. Combined effects of cadmium and decabrominated diphenyl ether on thyroid hormones in rats. Arh Hig Rada Toksikol, 2012; 63:255–62. PMid: 23152375. https://doi.org/10.2478/10004-1254-63-2012- 2179
- Randa AH, Dawlat MA, Nariman AR, Hatem ME, Dessouky MI. Clinicopathological, histopathological and immunological studies on animals exposed to lead and cadmium under experimental conditions. N Y Sci J. 2012; 5:12.
- Yongfeng Deng Y, Zhang Y, Lu Y, Zhao Y, Ren H. Hepatotoxicity and nephrotoxicity induced by the chlorpyrifos and chlorpyrifos-methyl metabolite, 3,5,6-trichloro-2-pyridinol, in orally exposed mice. Sci Total Environ. 2016; 544:507–14. PMid: 26674679. https://doi.org/10.1016/j.scitotenv.2015.11.162
- Manna P, Sinha M, Sil P. Amelioration of cadmium-induced cardiac impairment by taurine. Chem Biol Interact. 2008; 174(2):88–97. PMid: 18561905. https://doi.org/10.1016/j. cbi.2008.05.005
- Bharathi P, Gopala Reddy A, Rajasekher Reddy A, Alpharaj M. A study of certain herbs against chlorpyrifos-induced changes in lipid and protein profile in poultry. Toxicol Int. 2010; 18(1):44–6. PMid: 21430920 PMCid: PMC3052583. https://doi.org/10.4103/0971-6580.75854
- Snedecor GW, Cochran WG. Statistical methods, 8th ed. IOWA State University Press: Amer, IOWA, USA; 1994. p. 217–68.
- Tomaszewska E, Winiarska-Mieczan A, Dobrowolski P. Hematological and serum biochemical parameters of blood in adolescent rats and histomorphological changes in the jejunal epithelium and liver after chronic exposure to cadmium and lead in the case of supplementation with green tea vs black, red or white tea. Exp Toxicol Pathol. 2015; 67(5):331–9. PMid: 25837382. https://doi. org/10.1016/j.etp.2015.02.005
- Babaknejad N, Moshtaghie AA, Shahanipour K, Bahrami S. The protective roles of zinc and magnesium in cadmiuminduced renal toxicity in male Wistar rats. Iran J Toxicol. 2015; 8(27):1160–7.
- Ambali S, Joseph O, King A, Esievo N, Samuel A. Hemotoxicity induced by chronic chlorpyrifos exposure in Wistar rats: Mitigating effect of Vitamin C. Vet Med Int. 2010; 1–7. PMid: 21647348 PMCid: PMC3103876. https://doi. org/10.4061/2011/945439
- Barski D, Spodniewska A. Effect of chlorpyrifos and enrofloxacin on selected enzymes in rats. Pol J Vet Sci. 2018; 21(1):39–46..